Anglický abstrakt:
Amaryllidaceae are known as ornamental plants, furthermore some species of this family contain galanthamine, an acetylcholinesterase inhibitor approved for the treatment of Alzheimer´s disease, and other alkaloids with interesting pharmacological activity. The chemical composition of alkaloids from Zephyranthes grandiflora Lindl. was analyzed by GC/MS. Seven known compounds, belonging to five structural types of Amaryllidaceae alkaloids, were identified. The alkaloid extract from the bulbs showed promising cholinesterase inhibitory activities against human blood acetylcholinesterase (HuAChE; IC50 39.2±3.0 μg/mL) and human plasma butyrylcholinesterase (HuBuChE; IC50 356±9.3 μg/mL).

Anglický abstrakt:
The underivatized alkaloid mixture extracted from the bulbs of Chlidanthus fragrans Herb. was investigated by capillary GC/MS for the first time. Fifteen known Amaryllidaceae alkaloids of five structure types were identified. The main alkaloids were tazzetine (9, tazettine-type), chlidanthine (2, galanthamine-type), belladine (8, belladine-type) and lycorine (12, lycorine-type). The alkaloid extract from the bulbs showed promising human blood acetylcholinesterase (IC50 = 20.1 ± 2.9 μg/mL) and human plasma butyrylcholinesterase (IC50 = 136.8 ± 6.9 μg/mL) inhibitory activity.

Anglický abstrakt:
Tubers of Corydalis cava were extracted with ethanol and fractionated using n-hexane, chloroform and ethanol.Repeated column chromatography, preparative TLC and crystallization led to the isolation of fifteen isoquinoline alkaloids. The chemical structures of the isolated compounds were determined on the basis of spectroscopic techniques and by comparison with literature data. All isolated compounds were tested for human blood acetylcholinesterase (HuAChE) and human plasma butyrylcholinesterase (HuBuChE) inhibitory activity. (+)-Canadaline inhibited acetylcholinesterase as well as butyrylcholinesterase in a dose-dependent manner with IC50 values of 20.1 ± 1.1 μM and 85.2 ± 3.2 μM, respectively. (+)-Canadine, with an IC50 value of 12.4 ± 0.9 μM, was the most potent inhibitor of acetylcholinesterase, whilst (±)-corycavidine and (+)-bulbocapnine were effective inhibitors of butyrylcholinesterase with IC50 values of 46.2 ± 2.4 μM and 67.0 ± 2.1 μM, respectively. The other isolated alkaloids were considered inactive (IC50 > 100 μM).

V rámci hledání potencionálních inhibitorů acetylcholinesterasy (AChE) a butyrylcholinesterasy (BuChE), které hrají klíčovou úlohu v terapii Alzheimerovi nemoci, byla vybráno bioautografickou metodou dle Hosttetmanna Eschscholzia californica (Papaveraceae), kterávykazoval slibnou aktivitu. Z Eschscholzia californica bylo izolováno 14 látek, které byly testovány na inhibici AChE a BuChE). Pouze 2 izolované látky vykazovaly slibnou inhibiční aktivitu vůči lidské BuChE.