Offer

- Development and testing of new methods and instrumentation for microscale separation

- Expertise in the area of microscale separation techniques

- Cooperation with partners on development and construction of electrochemical detectors for microscale separation techniques

- Cooperation on development of electrophoretic strategies for rapid determination of various organic molecules in clinical samples (usable in various pharmacological and physiological studies)

- Cooperation with technological companies and research groups in this field

Know-how & Technologies

- New methodologies based on electromigration for determination of analytes in clinical and other environmental matrices

- Very short analysis time, very little sample volume is required

- Development of instrumentation for electrochemical detection and sample introduction in microscale separation techniques

Research Area & Excelence

- Development of new electrophoretic strategies for rapid determination of amino acids, saccharides and other metabolites in various clinical samples that represents background for solution of different pharmacological and physiological studies

- Construction and optimization of electrochemical detectors for microscale separation techniques

- Development of new preconcentration techniques of biological samples that are based on electrophoretic principle

Key Research Equipment

- Agilent 7100 Capillary Electrophoresis System and HP3D Capillary Electrophoresis System (Agilent Technologies) equipped with diod-array detector, contactless conductivity detector, fluorescence detector ARGOS 250B (Flux Instruments) and mass spectrometry detector CE/MS Single Quad ES Superior Line Bundle (Agilent Technologies)

- Microchip electrophoresis with a lab-build contactless detector

- Fully equipped biochemical laboratory for preparation and processing of the samples

Achievements

Technique of sampling low volume analytes (as low as 1 µl) is currently in the process of intellectual property protection.

Partnerships & Collaborations

Department of Analytical Chemistry, Faculty of Science, Charles University in Prague | J. Heyrovský Institute of Physical Chemistry | Institute of Physics | Department of Sport Medicine, Third Faculty of Medicine, Charles University in Prague | 2nd Internal Department of Third Faculty of Medicine and Faculty Hospital Královské Vinohrady | Centre for Research on Diabetes, Metabolism and Nutrition, Charles University in Prague | Department of Pharmacology, Third Faculty of Medicine, Charles University

Important Publications

- Tůma P., Opekar F., Jelínek I.: A Contactless Conductometric Detector with Easily Exchangeable Capillary for Capillary Electrophoresis, Electroanalysis 2001, 13, 989–992. http://dx.doi.org/10.1002/1521-4109(200108)13:12<989::AID-ELAN989>3.0.CO;2-C, IF2001 1,702, 59 times cited.

- Tůma P., Opekar F., Štulík K.: Contactless conductivity detector for capillary zone electrophoresis - effect of the detection cell geometry on the detector performance, Electrophoresis 2002, 23, 3718–3724. http://dx.doi.org/10.1002/1522-2683(200211)23:21<3718::AID-ELPS3718> 3.0.CO;2-U, IF2002 4,325, 52 times cited.

- Tůma P., Samcová E., Andělová K.: Determination of free amino acids and related compounds in amniotic fluid by capillary electrophoresis with contactless conductivity detection, J. Chromatogr. B 2006, 839, 12–18. http://dx.doi.org/10.1016/j.jchromb.2005.12.020, IF2006 2,647, 52 times cited.

- Tůma P., Samcová E., Opekar F., Jurka V. and Štulík K.: Determination of 1-methylhistidine and 3-methylhistidine by capillary and chip electrophoresis with contactless conductivity detection, Electrophoresis 2007, 28, 2174–2180. http://dx.doi.org/10.1002/ elps.200600697, IF2007 3,609, 23 times cited.

- Tůma P., Samcová E., Duška F.: Determination of Ammonia, Creatinine and Inorganic Cations in Urine using CE with Contactless Conductivity Detection, J. Sep. Sci. 2008, 31, 2260–2264. http:// dx.doi.org/10.1002/jssc.200700655, IF2008 2,746, 25 times cited.

- Tůma P., Málková K., Samcová E., Štulík K.: Rapid Monitoring of Arrays of Amino Acids in Clinical Samples Using Capillary Electrophoresis with Contactless Conductivity Detection, J. Sep. Sci. 2010, 33, 2394–2401. http://dx.doi.org/10.1002/jssc.201000137, IF2010 2,631, 31 times cited.

Are you interested in this expertise?

Please contact CPPT UK

Web: www.cppt.cuni.cz/

Mail: transfer@cuni.cz

Phone: +420 224 491 255

Experts and their department

Assoc. Prof. Ing. RNDr. Petr Tůma, Phd.

Department of Hygiene

Web: https://www.lf3.cuni.cz/3LFEN-325.html