Laboratory of Metabolism and Bioenergetics ****************************************************************************************** * ****************************************************************************************** [Page in Czech] [ URL "http://www.lf3.cuni.cz/3LF-1059.html"] Welcome to the Laboratory of Metabolism and Bioenergetics! Our laboratory focuses on mitochondrial function, cancer biology, and cardiovascular resea Mitochondrial Research: • Iron metabolism • Oxidative stress • Transport of substances across the mitochondrial membrane • Effects of drugs and environmental factors on mitochondrial function Cancer Biology: • Cultivation of organoids and 2D cell cultures from primary samples obtained from patient pancreatic adenocarcinoma • Personalized genotyping, metabotyping, and pharmacotyping of pancreatic tumors from pati • Investigation of tumor cell communication at the cellular level • Research on chemotherapeutic resistance Cardiovascular Research: • Pathophysiology of atrial fibrillation • Investigation of the molecular effects of new catheterization methods in arrhythmology • Gene transfection of cardiomyocytes Laboratory Activities • Cell and tissue cultures (organoids) • Confocal and fluorescence microscopy • Analysis of cellular respiration and metabolism • Flow cytometry • Protein and gene expression analyses • Electroporation of cell membranes • Fluoro/spectro/luminescence analyses • Statistical analyses in accordance with the principles of open science (Open Science), i experimental data and analytical scripts with a preference for Bayesian inference method Matlab) Our team: The head of the lab: prof. MUDr. Jan Trnka, Ph.D., M.Phil., M.Scjan.trnka@lf3.cuni.cz ORCID WebofScience · a graduate of general medicine at the 3rd Faculty of Medicine, Charles University, with studies in biochemistry and the history and philosophy of science at the University of Cam medical education at Karolinska Institutet in Stockholm · head of the Laboratory of Metabolism and Bioenergetics since 2010 and the Institute of B Cell, and Molecular Biology since 2017 · specializes in mitochondrial function and dysfunction, mitochondria-targeted drugs, tumo metabolism, and Bayesian modeling Assistant Professors: Ing. Stanislava Martínková, Ph.D.Podnadpis Text · a graduate of the doctoral program in Molecular and Cell Biology and Genetics at the Fac University of South Bohemia in České Budějovice · specializes in research on pancreatic ductal adenocarcinoma · focuses on improving targeted therapy and studying chemotherapeutic resistance in pancre · works with primary 2D and 3D cell cultures, cell lines, performs gene transfection, clon fluorescence microscopy, and measures metabolism, cellular respiration, and protein expres · Has experience in molecular and cell biology, biochemistry, including developmental and Mgr. Ivana Fišerová, Ph.D.Podnadpis Text · a graduate of Animal and Human Physiology at the Faculty of Science, Charles University, postgraduate degree in Human Pathophysiology at the 3rd Faculty of Medicine, Charles Unive · specializes in the pathophysiology of cardiovascular diseases · focuses on enhancing the safety and effectiveness of cardiac ablation for arrhythmogenic disorders · works with cell cultures, performs irreversible electroporation and electrotransfection, fluorescence microscopy, measures metabolism and protein expression, and analyzes and visu Matlab Researchers: Bc. Jana VorelPodnadpis Text · a graduate of the bachelor's program in Biochemistry and Biotechnology at the Faculty of Biochemical Technology, University of Chemistry and Technology, currently pursuing a maste Biochemistry · specializes in tumor biology metabolism · focuses on the influence of the cell cycle on cellular response to chemotherapeutic agen · works with cell cultures, pharmacotyping of cell lines and primary cultures, operates fl microscopy and flow cytometry, and measures metabolism and protein expression Postgraduate students: Mgr. Lucie Josefa LamačováPodnadpis Text · a graduate of the Organic Chemistry program at the Faculty of Science, Charles Universit · specializes in mitochondria-targeted compounds and iron metabolism · focuses on studying deferiprone derivatives targeted to mitochondria and their impact on in cancer cells · works with cell cultures, fluorescence microscopy, measures metabolism and protein expre MS, analyzes and visualizes data using R, conducts Bayesian modeling, and engages in scien Mgr. Michal ZuzčákPodnadpis Text · a graduate of Molecular Biology at the Faculty of Science, Comenius University in Bratis · specializes in the metabolism of pancreatic tumors · focuses on the regulatory mechanism of the lactate transporter MCT1 and the influence of function in pancreatic tumors · works with cell cultures, operates fluorescence microscopy and flow cytometry, and measu and protein expression MUDr. Mário BoďoPodnadpis Text · a graduate of general medicine from the 3rd Faculty of Medicine, Charles University, and the Internal Clinic of the FNKV and the 3rd Faculty of Medicine, Charles University. · specializes in the pathophysiology of pancreatic ductal adenocarcinoma. · focuses on researching the mechanisms of chemotherapeutic resistance in pancreatic ducta particularly on the development of resistance at low concentrations of cytotoxic drugs. · works with cell cultures, operates fluorescence microscopy, measures metabolism, cellula and protein expression. Undergraduate Students: Alice Kapáková David Kvapil Kateřina Šmejkalová Tomáš Wolf Alumni: Mgr. Anežka Kafková, Ph.D. Mgr. Zdeňka Syrová, Ph.D. Mgr. Jana Tůmová (Patková), Ph.D. (2009-2016) Moustafa Elkalaf, MBBCh, Ph.D. (2009-2019) Current Projects: • Pharmacotyping, metabotyping, genotyping, and research on chemotherapeutic resistance in ductal adenocarcinoma • 3/2024-12/2028 - Public competition of the ZEMĚ II Program – Ministry of Agriculture - Q Reproductive disorders in pigs - exposure of farms to harmful substances. Co-investigato Martínková • 2024/2026 - GAUK 108624 - Mechanisms of chemoresistance development in pancreatic adenoc concentrations of cytostatics. Principal Investigator: Mário Boďo • UNCE 24/MED/015 - Research on the influence of pathophysiological mechanisms in modern c disease treatment • Biophysical and safety aspects of pulsed field ablation (irreversible electroporation) o and surrounding tissue in vitro Unique Instruments: • Seahorse XFp and XF24 – real-time analysis of cellular respiration and anaerobic glycoly • TONAGENA electroporation system for electroporation and electrotransfection of adherent Collaborations: • MRC Mitochondrial Biology Unit, University of Cambridge • Internal Clinic of the 3rd Faculty of Medicine, Charles University, University Hospital Vinohrady • Cardiology Clinic of the 3rd Faculty of Medicine, Charles University, University Hospita Vinohrady • Internal Clinic of the 3rd Faculty of Medicine, Charles University, Bulovka Hospital • Institute of Histology and Embryology, Faculty of Medicine, Masaryk University • Faculty of Biomedical Engineering, Czech Technical University in Prague • Vera Vávrová's Laboratory, Pediatric Clinic of the 2nd Faculty of Medicine, Charles Univ Motol • Institute of Animal Production, Department of Reproductive Biology – Regulation of Mamma Maturation • Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Laboratory of Bi Molecular Biology of Germ Cells • Tonagena Ltd. Recent Publications: Zloh, Miloslav, Patrik Kutilek, Jan Hejda, Ivana Fiserova, Jan Kubovciak, Masaaki Murakami Stofkova. 2024. “Visual Stimulation and Brain-Derived Neurotrophic Factor (BDNF) Have Prot Effects in Experimental Autoimmune Uveoretinitis.” Life Sciences 355 (October). https://do j.lfs.2024.122996 [ URL "https://doi.org/10.1016/j.lfs.2024.122996"] Acimovic I, Gabrielová V, Martínková S, Eid M, Vlažný J, Moravčík P, Hlavsa J, Moráň L, Ca Staňo P, Procházka V, Kala Z, Trnka J, Vaňhara P. (2024). Ex-vivo 3D cellular models of pa adenocarcinoma: from embryonic development to precision oncology. Pancreas. 2024 Jul 30. d MPA.0000000000002393. Epub ahead of print. PMID: 39074056. Fiserova, Ivana, Ondrej Fiser, Marek Novak, Jan Trnka, Antonia Gibalova, David Kvapil, Bar Marek Hozman, Dalibor Herman, Klara Benesova, and Pavel Osmancik. 2024. “Significant Hemol Present during Irreversible Electroporation of Cardiomyocytes in Vitro.” Heart Rhythm. doi j.hrthm.2024.08.019. Osmancik, Pavel, Barbora Bacova, Dalibor Herman, Marek Hozman, Ivana Fiserova, Sabri Hasso Melenovsky, Jakub Karch, Jana Vesela, Klara Benesova, and Vivek Y. Reddy. 2024. “Periproce Intravascular Hemolysis During Atrial Fibrillation Ablation: A Comparison of Pulsed Field Radiofrequency Ablation.” JACC: Clinical Electrophysiology 10(7):1660–71. doi: 10.1016/j.j Lucie J. Lamačová, and Jan Trnka. 2024. “Chelating Mitochondrial Iron and Copper: Recipes, and Promise.” Mitochondrion. Elsevier B.V. https://doi.org/10.1016/j.mito.2024.101903 [ UR doi.org/10.1016/j.mito.2024.101903"] . Žalmanová T, Hošková K, Prokešová Š, Nevoral J, Ješeta M, Benc M, Yi YJ, Moravec J, Močáry S, Fontana J, Elkalaf M, Trnka J, Žáková J, Petr J. (2023). The bisphenol S contamination in human follicular fluid affects the development of porcine oocytes. Front Cell Dev Biol. doi: 10.3389/fcell.2023.1145182. PMID: 37091980; PMCID: PMC10115966. Anežka Kafková, Lisa Tilokani, Filip Trčka, Veronika Šrámková, Marie Vancová, Tomáš Bílý, Julien Prudent, and Jan Trnka. 2023. “Selective and Reversible Disruption of Mitochondrial Protein Complexes by Lipophilic Cations.” Mitochondrion 68 (January):60–71. https://doi.or j.mito.2022.11.006 [ URL "https://doi.org/10.1016/j.mito.2022.11.006"] . Fiserova I, Trinh MD, Elkalaf M, Vacek L, Heide M, Martinkova S, Bechynska K, Kosek V, Haj O, Tousek P, Polak J. (2022). Isoprenaline modified the lipidomic profile and reduced ?-ox cardiomyocytes: In vitro model of takotsubo syndrome. Front Cardiovasc Med. Aug 22;9:91798 fcvm.2022.917989. PMID: 36072861; PMCID: PMC9441769. Michal Šíma, Stanislava Martínková, Anežka Kafková, Jan Pala, and Jan Trnka. 2022. “Cell-T Interferes With DNA-Based Normalization of Metabolic Flux Data.” Physiological Research 71 26. https://doi.org/10.33549/physiolres.934855 [ URL "https://doi.org/10.33549/physiolres. Michal Zuzčák, and Jan Trnka. 2022. “Cellular Metabolism in Pancreatic Cancer as a Tool fo Treatment (Review).” International Journal of Oncology. Spandidos Publications. https://do IJO.2022.5383. J. Fontana, S. Martínková, J. Petr, T. Žalmanová, J. Trnka. Metabolic cooperation in the o Physiol. Res. 69: 33-48, 2020; doi: 10.33549/physiolres.934233 Moráň Lukáš; Pivetta Tiziana; Masuri Sebastiano; Vašíčková Kateřina; Walter Franziska; Pre Jochen; Elkalaf Moustafa; Trnka Jan; Havel Josef; Vaňhara Petr. Mixed copper(ii)-phenanthr complexes induce cell death of ovarian cancer cells by evoking the unfolded protein respon Metallomics,11,9,1481-1489 Moráň, Lukáš, Tiziana Pivetta, Sebastiano Masuri, Kateřina Vašíčková, Franziska Walter, Jo Prehn, Moustafa Elkalaf, Jan Trnka, Josef Havel, and Petr Vaňhara. 2019. “Mixed Copper(Ii) Complexes Induce Cell Death of Ovarian Cancer Cells by Evoking the Unfolded Protein Respon 11 (9): 1481–89. https://doi.org/10.1039/c9mt00055k.